Insights into transcriptional expression and putative functions of multiple polyhydroxyalkanoate synthase paralogs in Haloferax mediterranei.

ABSTRACT: The halophilic archaeon Haloferax mediterranei is a promising candidate for polyhydroxyalkanoate (PHA) production, offering several advantages due to its extremophilic physiology. While its primary PHA synthase, a class III enzyme composed of PhaCHme and PhaEHme subunits, has been well characterized, the genome encodes three additional phaC paralogs (phaC1, phaC2, and phaC3), which were previously labeled as cryptic and remain poorly understood. In this study, we systematically investigated these paralogs by employing a targeted bioinformatics pipeline, revealing notable diversity in PHA synthases among Halobacteriales and underscoring the distinctiveness of H. mediterranei. We further analysed the native transcriptional expression profiles of all phaC paralogs under three physiologically

SUBMITTER: Vanden Haute C 

PROVIDER: S-EPMC12863089 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature