A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy.

ABSTRACT: Protists represent a significant portion of eukaryotic diversity with a wide range of ecological roles, lifestyles, and diverse morphological traits. Despite their widespread importance in ecological systems and their potential as model organisms, most protist lineages remain poorly characterized, in part due to their small size and the fact that many lineages remain uncultivated. High-resolution microscopy techniques like transmission and scanning electron microscopy (TEM and SEM) are powerful tools for studying protists, but the application of these techniques has significant limitations when applied to uncultivated species, largely due to the absence of reproducible methodologies tailored to studying single cells with small sample sizes. We present a robust protocol for preparing indivi

SUBMITTER: Palka MV 

PROVIDER: S-EPMC12863111 | biostudies-literature | 2026 Mar-Apr

REPOSITORIES: biostudies-literature