In vitro wound simulation: A high-throughput device for scratch assays.

ABSTRACT: Traumatic injury to the healthy central nervous system (CNS) causes mechanical tissue damage that results in localized cell death and blood-brain-barrier (BBB) disruption. CNS tissue damage stimulates a multicellular wound response to limit the extent of damage but fails to reestablish the normal function of injured tissue. There is strong interest in developing new strategies to augment regeneration after CNS injury. To enable therapy development, reliable assays to screen and identify molecular approaches to augment glial-based wound responses over fibrotic scarring are needed. Scratch assays, which involve mechanically removing cells from an in vitro culture, allow for the simulation of wounds with high throughput and tight control over applied treatments to mechanistically study cell m

SUBMITTER: Labovitz JE 

PROVIDER: S-EPMC12863250 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature