Rapid Typing of Extended-Spectrum β-Lactamase (ESBL)- and Metallo-β-Lactamase (MBL)-Producing Enterobacterales Using Fourier Transform Infrared (FT-IR) Spectroscopy.

ABSTRACT: This study investigated the usefulness of the IR Biotyper, which types bacterial strains using Fourier transform infrared (FT-IR) spectroscopy, against extended-spectrum β-lactamase (ESBL)- and metallo-β-lactamase (MBL)-producing Enterobacterales. Sixty-six clinical isolates (20 ESBL-producing Klebsiella pneumoniae, 15 IMP-producing K. pneumoniae, and 31 IMP-producing Enterobacter cloacae complex isolates) were analyzed using the IR Biotyper, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and whole-genome single-nucleotide polymorphism (wgSNP) analyses and the results were compared. Of the 20 ESBL-producing K. pneumoniae strains analyzed, the IR Biotyper detected three clusters. Of these clusters, two were determined as respective clusters by PFGE and wgSNP ana

SUBMITTER: Kawamoto Y 

PROVIDER: S-EPMC12863417 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature