Loss of IL1RA promotes prostate cancer growth and metastasis by activating Akt signaling pathway.

ABSTRACT:

Background

Interleukin-1 receptor antagonist (IL1RA) blocks the interaction between IL-1 and its receptors. It modulates inflammatory responses, cell proliferation, and the invasion of cancer cells. In this study, we examined the biological functions of IL1RA and the mechanisms that influence its effects on prostate cancer (PCa).

Materials and methods

We performed RT-qPCR and Western blot analyses to evaluate IL1RA expression levels in various cell lines. For functional studies, we employed MTT, colony formation, soft agar, wound healing, and transwell migration and invasion assays. Then, we analyzed Western blots to elucidate the underlying mechanisms involved. Xenograft mouse models were ultimately established after the overexpression of IL1RA.

Results

IL1RA was exp

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12863537 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature