Bioassay-guided fractionation and identification of wound healing active compounds from Khaya senegalensis leaves.

ABSTRACT: This study investigated the wound healing potential of Khaya senegalensis leaves through a comprehensive approach, including phytochemical, antioxidant, cytotoxicity, migration, and molecular docking analyses. Bioassay-guided isolation identified β-sitosterol and stigmasterol, with structures confirmed by NMR and FTIR analyses. Phytochemical screening revealed the presence of flavonoids, saponins, triterpenoids, tannins, and steroids, with solvent polarity significantly influencing composition. The ethyl acetate extract exhibited the strongest DPPH radical scavenging (IC₅₀ = 5.6 mg/mL). The methanol extract had the highest total phenolic content (198.8 mg GAE/g) while the ethyl acetate had the highest flavonoid content (1,228.1 mg QE/g). MTT assays confirmed the cytocompatibility of all ex

SUBMITTER: Mohd Khamil AN 

PROVIDER: S-EPMC12863557 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature