Transcriptomic and ultrastructural responses to Amiodarone-Itraconazole in naturally benznidazole-resistant and -susceptible Trypanosoma cruzi strains.

ABSTRACT: Chagas disease (CD), caused by Trypanosoma cruzi, remains a major therapeutic challenge, primarily due to the limited efficacy of benznidazole and the emergence of naturally resistant strains. In this context, drug repurposing offers a promising strategy to identify compounds with trypanocidal activity. In this study, we evaluated the effect of Amiodarone-Itraconazole (Amiozole) against two T. cruzi strains belonging to DTU-TcI: one benznidazole-sensitive (MG) and one naturally resistant to benznidazol (DA). We employed an integrated approach combining transcriptomic and ultrastructural analyses to elucidate the compound's mechanisms of action. Trypanocidal activity was assessed through cell viability assays (MTT), and IC50 values were determined using epimastigotes cultured in LIT medium.

SUBMITTER: Gutierrez S 

PROVIDER: S-EPMC12863684 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature