Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial integration.

ABSTRACT: Kidney organoids derived from human pluripotent stem cells have emerged as promising models for studying kidney disease and therapeutic development. However, the lack of a scalable production system has limited their industrial applications in regenerative medicine. Here, we have developed a cost-effective mass-production method for manufacturing vascularized kidney organoids, which has improved production efficiency by more than 50 times compared to conventional culture systems. The incorporation of a dynamic culture environment in delta-wing stirred bioreactors has significantly enhanced the glomerular vascularization of kidney organoids via mechanosensory integrin α2β1. Single-cell RNA sequencing and functional analyses demonstrated the enhanced maturation in STR nephron epithelia. The

SUBMITTER: Tekguc M 

PROVIDER: S-EPMC12864042 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature