Genome mining and functional characterization of four type I sesquiterpene synthases from the Tiger Milk Mushroom Lignosus rhinocerus.

ABSTRACT: The Tiger Milk mushrooms (Lignosus spp.) have long been used as traditional folk medicines throughout Southeast Asia. However, the chemical constituents of these species remain largely unexplored. In this study, four type I sesquiterpene synthases (LrhTS1-LrhTS4) from Lignosus rhinocerus TM02^® were functionally characterized through genome mining, in vitro enzymatic assays, and heterologous expression in an Escherichia coli host engineered to overexpress an exogenous mevalonate pathway. The enzyme products were analyzed by GC-MS and further purified and structurally elucidated by NMR spectroscopy. A total of 11 sesquiterpenes (1-11) were identified, among which two were unstable and converted to stable derivatives (2 to 2a and 7 to 7a). Notably, compounds 1-10 are reported from

SUBMITTER: Gao MX 

PROVIDER: S-EPMC12864622 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature