<i>Ganoderma lucidum</i> polysaccharide-decorated extracellular vesicle enables synergistical antitumor immunotherapy.

ABSTRACT: Ganoderma lucidum polysaccharide (GLP) holds considerable promise for tumor therapy, but its clinical application is limited by its poor tumor-targeting capability. Herein, we report the development of an immunoregulatory nano-bioconjugate formed by conjugating GLPs with low-pH culture medium reprogrammed CT26 tumor cell-derived extracellular vesicle (LTEV). Specifically, 4-carboxybenzeneboronic acid (CPBA), used as an intermediate coupling agent, is conjugated with the amino group of LTEV via its carboxyl group to obtain CPBA-LTEV. GLP is then covalently linked via boric ester linkages between its hydroxyl groups and the boronic acid groups of CPBA. After systemic administration, GLP@LTEV accumulates in homologous tumor tissues derived from the LTEV parent cells, remodeling the imm

SUBMITTER: Ouyang Z 

PROVIDER: S-EPMC12864664 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature