Ultrasound stimulation increases cartilage synthesis and revert dedifferentiation on human chondrocytes.

ABSTRACT: Therapeutic ultrasound (US) holds promise as a potential therapy to treat articular cartilage; however, optimized and targeted US protocols are lacking. This study aimed at determining an appropriate set of US protocols for stimulating human chondrocyte activity. Human chondrocytes were exposed to various US parameters, including different central frequencies, power densities, operation modes, pulse parameters, and durations, either daily or every other day over a three-day period. Temperature was monitored during stimulation. Protocols that showed the greatest potential for increasing chondrocyte metabolic activity and proliferation were further investigated and extended to a seven-day application. Cartilage matrix synthesis was analyzed by immunocytochemistry, qPCR, and glycosaminoglycan

SUBMITTER: Oliveira S 

PROVIDER: S-EPMC12864824 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature