A high diversity naive variable new antigen receptor, vNAR, phage library for rapid nanobody discovery across diverse antigens.

ABSTRACT: Conventional antibodies are among the most frequently used and effective biological tools explored for therapeutic and diagnostic applications. However, they face significant limitations when it comes to challenges that demand specialized attributes such as rapid tissue penetration, the ability to bind to concealed epitopes, and stability in nonphysiological environments. In recent years, shark-derived immunoglobulin variable new antigen receptor (vNAR) has emerged as a promising alternative to overcome these limitations. In this study, we constructed a naïve vNAR phage display library from a white-spotted bamboo shark (Chiloscyllium plagiosum), with a library diversity size of ∼3 × 10¹¹ clones. Next generation sequencing analysis revealed the high diversity of the library, allo

SUBMITTER: Kumar V 

PROVIDER: S-EPMC12865624 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature