Both obesogenic and isocalorically-controlled diets drive changes in microbiota composition in a sex-dependent manner in mice.

ABSTRACT: We have observed the loss of structure and function of olfactory circuits following consumption of excess dietary fat as maintained by isocaloric feeding. Because changes in microbiome composition have not been examined during this type of diet that does not induce overt obesity, we performed isocaloric feeding of a moderately high-fat (MHF) diet, and assessed gut microbiome changes over 5 months by sequencing the 16s V4 region in fecal samples. MHF diet similarly reduced alpha diversity in both isocaloric and ad libitum fat-fed animals by 5 months. Bray-Curtis dissimilarity analysis revealed unique beta diversity in each dietary group in female mice at 3 and 5 months, while fat-fed groups of male mice were not different. Elevated abundance of Firmicutes was found in all MHF ad libitum fed

SUBMITTER: Loeven AM 

PROVIDER: S-EPMC12865689 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature