A new crystal form of the DNA-free full-length XPD helicase from Thermoplasma acidophilum.

ABSTRACT: The XPD helicase plays a critical role in DNA repair and serves as a model for structural studies of superfamily 2 (SF2) helicases. We report a novel orthorhombic crystal form of DNA-free Thermoplasma acidophilum XPD (TaXPD) obtained under high ionic strength conditions generated by sodium potassium tartrate and NaCl-based vapor-diffusion conditions, in contrast to earlier previously reported conditions that used polyols (PEG) or diols (MPD). The crystals belonged to space group P2₁2₁2₁ (a = 59.53, b = 96.00, c = 159.09 Å) and diffracted to 2.13 Å resolution, yielding the highest resolution TaXPD structure to date. Structural analysis showed that this crystal form contains fewer intermolecular interfaces than the previously reported hexagonal lattice, as su

SUBMITTER: Bravo M 

PROVIDER: S-EPMC12865780 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature