DNA methylation landscapes in human cells and their chromatin determinants.

ABSTRACT:

Background

DNA methylation patterns are established during development and are propagated in a cell type specific manner, but these patterns may become aberrant during aging and cancer. Regions of alternating high and moderate to low levels of DNA methylation exist along all chromosomes in human cells. It is unclear how these distinct DNA methylation blocks are established. Most of the prior work in this area has been performed with mouse embryonic stem cells.

Methods

Using whole genome bisulfite sequencing and chromatin-immunoprecipitation sequencing, we have profiled DNA methylation at single base resolution and various histone modifications in human bronchial epithelial cells.

Results

We found that many regions of lower DNA methylation (< 50%) are characterized by

SUBMITTER: Cui W 

PROVIDER: S-EPMC12865891 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature