Unique Aerofoil-Structured Microfluidics for High Throughput Lipid Nanoparticle Formulation Screening and Scale-up.

ABSTRACT: Herein, a high-throughput microfluidic platform is presented that addresses the challenges in lipid nanoparticles (LNPs) formulation screening and scale-up through the design of micromixers with unique aerofoil structures. These structures are optimized to enhance mixing and LNP formation across a wide flow rate range (0.2-50 mL min^-1), using minimal reagent volumes (as low as 0.1 mL) up to liter-scale production. A high-throughput formulation screening instrument named MiNANO-form and a microfluidic cartridge with eight parallel channels is introduced, which enables simultaneous synthesis of multiple LNP formulations with excellent quality (size 38-150 nm and polydispersity index (PDI), PDI < 0.2) and reproducibility. In addition, for a seamless scale-up, the platform consists

SUBMITTER: Liu D 

PROVIDER: S-EPMC12866714 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature