A Patient-Derived Organoid Biobank of Adamantinomatous Craniopharyngioma as a Platform for Drug Discovery.

ABSTRACT: Adamantinomatous craniopharyngioma (ACP), a benign yet clinically challenging neoplasm situated in sellar-suprasellar region, frequently causes hypothalamic dysfunction. Despite the identification of molecular alterations in ACP, the absence of robust research models has impeded the advancement of targeted therapies. Herein, the development of a large-scale ACP biobank comprising 54 patient-derived organoids (PDOs) is presented, achieves with a notable 90% success rate. Comprehensive characterization using hematoxylin and eosin (H&E) staining, immunofluorescence staining, and whole-exome sequencing (WES) demonstrates that PDOs faithfully recapitulate key histoarchitectural features, molecular marker expression profiles, and somatic mutational landscapes of corresponding parental tumors. Dr

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12866856 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature