A highly resolved integrated single-cell atlas of human breast cancers.

ABSTRACT: In this study, we developed an integrated single-cell transcriptomic (scRNAseq) atlas of human breast cancer (BC), the largest resource of its kind, totaling >600 000 cells across 138 patients. Rigorous integration and annotation of publicly available scRNAseq data enabled a highly resolved characterization of epithelial, immune, and stromal heterogeneity within the tumor microenvironment (TME). Within the immune compartment, we were able to characterize heterogeneity of CD4, CD8 T cells, and macrophage subpopulations. Within the stromal compartment, subpopulations of endothelial cells (ECs) and cancer-associated fibroblasts (CAFs) were resolved. Within the cancer epithelial compartment, we characterized the functional heterogeneity of cells across the axes of stemness, epithelial-mesenchy

SUBMITTER: Chen A 

PROVIDER: S-EPMC12867518 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature