RNA-seq analysis reveals a positive role for NGF in the myogenic differentiation of bovine skeletal muscle satellite cells.

ABSTRACT: In this study, we successfully isolated and cultured bovine skeletal muscle satellite cells (bSMSCs) and induced muscle cell formation in vitro. Skeletal muscle satellite cells (SMSCs) were isolated from the deep tissues of foetal bovine hind limbs and differentiated with 2% horse serum in vitro. The transcriptome sequencing results revealed a total of 1030 differentially expressed genes (DEGs) in the middle stage of differentiation (day 3) compared with the predifferentiation stage (day 0). A total of 374 DEGs were identified in the postdifferentiation stage (day 7) compared with the middle differentiation stage (day 3). We further investigated the regulatory effects of the DEG nerve growth factor (NGF) on the proliferation and myogenic differentiation of bSMSCs. The overexp

SUBMITTER: Li X 

PROVIDER: S-EPMC12867674 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature