Soluble adenylyl cyclase in nonmammalian sperm is directly controlled by pH, not by HCO₃^- or Ca².

ABSTRACT: In mammalian sperm, high HCO₃^- concentrations in semen and the oviduct activate soluble adenylyl cyclase (sAC), which synthesizes cyclic AMP to regulate sperm motility. Here, we demonstrate that sAC orthologs in echinoderms and fish, species whose sperm fertilize eggs in aquatic environments with low HCO₃^-, are activated by alkaline pH rather than HCO₃^-. In human sAC, two charged residues coordinate HCO₃^- and are essential for HCO₃^--mediated activation. In contrast, pH-regulated sACs and orthologs from 13 phyla have these residues replaced by neutral ones. These substitutions abolish the enzyme's responsiveness to HCO₃^- and suggest that pH regulation of sAC is

SUBMITTER: Kendall O 

PROVIDER: S-EPMC12867704 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature