Intronic polyadenylation-derived long noncoding RNA modulates nucleolar integrity and function.

ABSTRACT: RNAs transcribed from protein-coding gene loci are widely assumed to be translated into proteins. However, intronic polyadenylation (IPA) occurring near the transcription start site or within early introns can generate noncoding RNAs derived from protein-coding loci. Despite their abundance, the functional roles of such RNAs remain largely unexplored. In this study, we investigated one such noncoding RNA, CUL1-IPA, transcribed from the CUL1 gene locus. Our study revealed that CUL1-IPA is an RNA polymerase II-dependent IPA isoform that is polyadenylated, stable, and translocates to the nucleolus. Functional characterization demonstrated CUL1-IPA to play a critical role in maintaining nucleolar integrity. RNA-protein interaction assay identified GPATCH4 and NOP58, nucleo

SUBMITTER: Mallick S 

PROVIDER: S-EPMC12867753 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature