Genetic identification of mutations and MLST types associated with decreased susceptibility to ceftriaxone in <i>Neisseria gonorrhoeae</i>.

ABSTRACT: Antimicrobial resistance (AMR) in Neisseria gonorrhoeae severely limits treatment options, with increasing resistance even to first-line and last-line ceftriaxone (CRO), posing a major global public health threat. In this study, we systematically identified 53 significantly different mutations between ceftriaxone-resistant and susceptible strains in multiple proteins through bioinformatics analysis. Among these, 33 mutations were identified for the first time, notably including the PorB Q143K via structural analysis. Minimum spanning tree (MST) analysis based on these mutations marked improved sensitivity and specificity for identifying ceftriaxone-resistant strains compared to traditional sequence typing of PenA, PonA, PorB, and MtrR (68.4% vs. 53.2%; 77.3% vs. 57.5%, respectively)

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12868221 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature