Genome-wide identification and functional validation of RLCK VII subfamily genes conferring disease resistance in broad bean (<i>Vicia faba</i> L.).

ABSTRACT:

Introduction

Members of receptor like cytoplasmic kinase VII (RLCK VII) subfamily are important participants in plant growth and development, innate immunity, and resistance to abiotic stress. However, in broad beans, the regulatory mechanisms of RLCK VII subfamily genes involved in these processes remains unclear.

Methods

To further elucidate the regulatory mechanisms, a comprehensive whole-genome analysis was conducted. To investigate the disease resistance function of VfRLCK VII genes, their expression patterns under infection by Alternaria alternata were analyzed through transcriptome sequencing. And functional validation of VfRLCK VII4 (VfRLCK176) was performed via transformation into Nicotiana tabacum (tobacco).

Results

VfRLCK VII sub

SUBMITTER: Wang F 

PROVIDER: S-EPMC12868224 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature