Microgravity-induced transcriptional reprogramming in embryonic chicken limb bud-derived chondrogenic cultures.

ABSTRACT:

Introduction

Extended exposure to microgravity, such as experienced during spaceflight, significantly alters the mechanical environment of skeletal tissues, impacting cartilage development and function. Mechanical unloading disrupts the balance of cellular signaling and extracellular matrix synthesis in cartilage precursor cells, but the molecular consequences and temporal dynamics of these alterations remain incompletely understood.

Methods

We employed simulated microgravity via a random positioning machine (RPM) to investigate stage-specific transcriptomic and phenotypic responses in chondrogenic micromass cultures derived from embryonic chicken (Gallus gallus) limb bud cells. RNA sequencing, bioinformatic pathway analysis, and protein interaction network constructi

SUBMITTER: Kovacs P 

PROVIDER: S-EPMC12868230 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature