Evolution and phylogenetic characteristics of the first <i>Brucella canis</i> strain isolated from a human patient in Yunnan Province, China.

ABSTRACT:

Introduction

Brucella canis is a zoonotic pathogen that infects both dogs and humans, yet its evolutionary and phylogenetic characteristics are poorly understood.

Methods

Here, we comprehensively characterized an isolated strain of B. canis through integrated bacteriological, comparative genomic, and whole-genome sequencing-based core genome single-nucleotide polymorphism (WGS-cgSNP) analyses.

Results

B. canis YN20042 was isolated from a febrile patient (38 °C) with sweating and fatigue. The culture exhibited rough, grayish white, sticky, and opaque colonies. The isolate was identified as Brucella strain by a BCSP-31 polymerase chain reaction (PCR) assay, which yielded an amplicon of the expected 223-bp size, and was classified as a B. c

SUBMITTER: Yang Q 

PROVIDER: S-EPMC12868276 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature