Ecto-5'-nucleotidase/CD73 reduces COX-2 expression in activated macrophages.

ABSTRACT: Extracellular ATP (eATP) is a significant contributor of inflammation through enhanced expression of cyclooxygenase 2 (COX-2). In this study, we explored whether overexpression of ectonucleotidases such as ecto-5'-nucleotidase (NT5E, or CD73), responsible for the hydrolysis of AMP to adenosine, can modulate COX-2 expression in macrophages. CD73 was subcloned in both bacterial and mammalian expression vector systems. In the first model, recombinant CD73 was exogenously added into the media of J774A.1 macrophage cells co-stimulated with LPS and AMP. In the second model, J774A.1 cells were transfected with CD73 cloned in pcDNA3.1 vector and checked for the expression of COX-2 following treatment with LPS and AMP. In both models, CD73 activity was assessed by the release of inorganic phosphate

SUBMITTER: Rahman MM 

PROVIDER: S-EPMC12868784 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature