Brain transcriptome analysis reveals novel long non-coding RNAs in Dryophytes arenicolor (Canyon Treefrog).

ABSTRACT: Anurans possess complex genomes, making molecular research challenging, particularly in non-model species. Transcriptomics offers a powerful tool for uncovering genomic responses to environmental changes through distinct transcriptional patterns. A sizeable portion of the transcriptome consists of non-coding RNAs, with long non-coding RNAs (lncRNAs) playing key roles in gene regulation. Here, we performed de novo transcriptome assembly from brain samples of Dryophytes arenicolor across 3 life stages: Pre-metamorphic, Metamorphic Climax, and Adults. By aligning our transcriptomes to LNCipedia, we identified 4,557 previously annotated lncRNAs with potential roles in gene regulation, macromolecule biosynthesis, and chromatin organization. To detect novel lncRNAs, we implemented a bioinformati

SUBMITTER: Herrera-Orozco H 

PROVIDER: S-EPMC12869070 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature