Fe-S cluster deficiency drives small colony variant formation in persistent infections.

ABSTRACT:

Introduction

Small colony variants (SCVs) of Staphylococcus aureus (S. aureus) are associated with persistent infections and poor clinical outcomes. The mechanisms driving stable SCV formation remain poorly understood, particularly concerning metabolic adaptations. This study explores the in-host evolutionary dynamics of S. aureus and identifies a novel genetic determinant linked to SCV formation.

Objectives

To investigate the genetic mutations and phenotypic adaptations underlying SCV formation, with a focus on the role of a novel mutation in the sufB gene, which is critical for Fe-S cluster biosynthesis.

Methods

Sequential isolates from a patient with recurrent infections were analyzed using whole-genome sequencing, antimicrobial susceptibility testing, and function

SUBMITTER: Chen T 

PROVIDER: S-EPMC12869195 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature