Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis.

ABSTRACT:

Introduction

Pericytes (PCs) are mural cells exhibiting some mesenchymal stem cell (MSC) properties and contribute to tissue regeneration after injury. We have previously shown that glioblastoma cancer cells induce in PCs, a pathogenic upregulation of chaperone-mediated autophagy (CMA) which modulates immune functions and MSC-like properties to support tumor growth.

Objectives

The aim of the study was to interrogate the role of CMA-regulated MSC properties in PCs in the context of tissue repair during inflammation triggered by a demyelinating injury.

Methods

Studies of RNA-seq were done PCs with (WT) and without (LAMP-2A KO) CMA. Cell characterization related to stemness, lineage and morphology was done in WT and KO PCs. Secretome analysis and cell differentiation ass

SUBMITTER: Salinas MD 

PROVIDER: S-EPMC12869254 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature