Aquaporin 4 and its isoforms regulation ameliorate AQP4 Mis-localization-induced glymphatic dysfunction in ischemic stroke.

ABSTRACT:

Introduction

The glymphatic system, a brain waste clearance pathway, is impaired during ischemic stroke-induced edema, although the underlying mechanisms remain unclear.

Objectives

This study investigates the temporal dynamics of glymphatic dysfunction post-stroke and the roles of aquaporin 4 (AQP4), its isoforms, and syntrophin alpha 1 (SNTA1) in AQP4 polarization.

Methods

Using a transient middle cerebral artery occlusion (tMCAO) mouse model, glymphatic function was assessed via cisterna magna contrast injection and magnetic resonance imaging. The AQP4 antagonist TGN-020 was administered to elucidate edema's role in glymphatic dysfunction. AQP4 isoforms viral vectors and SNTA1 modulation were used to study AQP4 polarization and glymphatic function. Techniques includ

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12869294 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature