Dynamic profiling of BMSC-dECM reveals accumulation of core matrisome proteins suppresses osteogenic differentiation and bone regeneration.

ABSTRACT:

Introduction

The extracellular matrix (ECM) of bone mesenchymal stem cells (BMSCs) plays a critical role in tissue development and regeneration. Rather than being inert, the ECM exhibits a dynamic structure that determines cell fate.

Objectives

The aim of this study is to investigate the dynamic composition, functional properties, and underlying mechanisms of BMSC-ECM during osteogenic differentiation. We propose that dynamic alterations in BMSC-ECM, particularly in critical matrix proteins, are essential to regulate osteogenic differentiation and bone regeneration.

Methods

Dynamic ECM from BMSCs was collected at different time points during culture with or without osteogenic induction, followed by decellularization. A mouse tibial defect model was introduced to asses

SUBMITTER: Li M 

PROVIDER: S-EPMC12869295 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature