Diverse Reagent Scaffolds Provide Differential Selectivity of 2'-OH Acylation in RNA.

ABSTRACT: RNA 2'-OH acylation is widely used both for mapping structure and for conjugating RNA, generally relying on selective reactions with unpaired nucleotides over paired ones. Common reagents for this acylation have been chiefly restricted to two similar aryl scaffolds, leaving open the question of how more broadly varied reagent structure might affect selectivity. Here, we prepared a set of 10 structurally diverse acylimidazole reagents and employed deep sequencing to profile their reactivity and selectivity in an RNA library of systematically varied structure. We show that structure-directed reactivity profiles vary significantly with the reagent scaffold, and we document new acylating agents that have altered selectivity profiles, including reagents that show elevated selectivity within loo

SUBMITTER: Xiao L 

PROVIDER: S-EPMC12870210 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature