PARP1 trapping activates cGAS-STING pathway to induce immunogenic cell death in multiple myeloma.

ABSTRACT:

Background

Alternative Non-Homologous End Joining (Alt-NHEJ) DNA repair is considered a major player in cancer genomic instability. Here, we investigated cGAS-STING pathway as crucial node in the interplay between Alt-NHEJ repair and immune response, in the aim to discover novel therapeutic vulnerability in Multiple Myeloma (MM).  METHODS: In silico analyses were performed by querying publicly available MM datasets (GSE66293 and CoMMpass). Anti-proliferative activity was evaluated by CellTiter-Glo, while flow cytometry analysis was used to determine the apoptotic process, cell cycle, phagocytosis, micronuclei detection, Calreticulin and T-cell activation markers. Protein expression was detected by western blot of whole or fractioned protein extracts.

Results

By interrogating

SUBMITTER: Juli G 

PROVIDER: S-EPMC12871033 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature