IRF1 Is an upstream regulatory transcription factor of eosinophils in type 1 environments.

ABSTRACT:

Background

Eosinophils have specific immune phenotypes in type 2 and type 1 environments. The regulatory transcription factors (TFs) that control eosinophil activation in type 2 or type 1 immune phenotypes E2 (eosinophils treated with IL-4, GM-CSF, IL-33, and IL-5) or E1 (eosinophils treated with IFN-γ, TNF-α, and IL-5), respectively, are unknown.

Objective

We sought to compare mouse and human eosinophil immune phenotypes following exposure to type 2 or type 1 polarizing cytokines and identify TFs that may regulate these responses.

Methods

Peripheral blood eosinophils were isolated from wild-type mice and from healthy human donors. Cells were cultured with type 2 (IL-4, GM-CSF, and IL-33) or type 1 (IFN-γ and TNF-α) cytokines. Cells underwent characterization of morph

SUBMITTER: Nazaroff CD 

PROVIDER: S-EPMC12871069 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature