Cell type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.

ABSTRACT: Intercellular communication is critical for maintaining organismal metabolic homeostasis. Here, we present a new method enabling temporally controlled, cell type-specific labeling of secreted and membrane proteins in key metabolic tissues. The method employs a genetically encoded proximity-labeling strategy by targeting a Cre-dependent TurboID ligase to the endoplasmic reticulum (ER) in ES cell-derived mice. Expression of TurboID in liver, adipose tissue, and spleen enabled the characterization of organ-specific ER proteomes at baseline and in response to fasting, inflammation, and dietary obesity, revealing tissue- and perturbation-specific changes and augmenting our understanding of how the proteomes of individual tissues change to regulate systemic energy balance. This comprehensive res

SUBMITTER: Plucinska K 

PROVIDER: S-EPMC12871138 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature