A new single chain, genetically encoded biosensor for RhoB GTPase based on FRET, useful for live-cell imaging.

ABSTRACT: RhoB is an atypical Rho GTPase whose function is tightly linked to its subcellular localization and membrane trafficking, reflecting its unique post-translational modifications and association with endosomal membranes in addition to the plasma membrane. Despite its implication in membrane trafficking and cytoskeletal regulation, tools to directly monitor RhoB activity in space and time have been lacking. Here, we describe the development and validation of a single-chain, genetically encoded Forster resonance energy transfer (FRET) biosensor that enables direct visualization of RhoB activity in living cells while preserving its native membrane-targeting determinants. The biosensor exhibits a large dynamic range and resolves spatially heterogeneous RhoB activity during leading-edge protrusio

SUBMITTER: Pagano S 

PROVIDER: S-EPMC12871158 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature