Decreased tRNA abundance contributes to decreased translation elongation rate in a prolonged mitosis.

ABSTRACT: Cells undergo dramatic structural rearrangements upon entering mitosis. In addition, the biochemistry of mitotic cells is dramatically altered, including a significant decrease in protein synthesis. The majority of studies of mitotic translation have used cells synchronized by cell cycle altering drugs in transformed cells and much less is known about mitotic translation in primary cells under native conditions. Previous work has found that mitosis activates the integrated stress response (ISR) to trigger eIF2α phosphorylation, but little is known about the input for this response. In this study, we focus on mitotic translational regulation in an immortalized, non-transformed cell line. We confirm decreased mitotic protein synthesis in primary cells and under native conditions. Additionall

SUBMITTER: Sparago E 

PROVIDER: S-EPMC12871220 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature