A Mammalian Surface Display Platform to Optimize the Antigenicity of Viral Proteins for Vaccine Design.

ABSTRACT: Vaccine development often involves modifying native viral proteins to enhance their stability and antigenicity, as seen in approved Covid-19 vaccines and multiple HIV vaccine candidates currently under investigation. High throughput screening on the surface of mammalian cells enables the rapid evaluation of oligomeric, glycosylated viral proteins and the identification of mutations that improve their properties for vaccine design. Here, we developed an experimental platform that uses the PiggyBac transposon system to display libraries of viral protein variants on the surface of mammalian cells for efficient screening. This approach addresses common challenges in existing mammalian display systems, including low transfection efficiency and the development of stable cell lines for ite

SUBMITTER: Christopher J 

PROVIDER: S-EPMC12871315 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature