Drying parameters and aging modulate protective properties of vitrified trehalose.

ABSTRACT: Storage of biological materials is essential for medical, research, and biotechnological applications. While cold-chain preservation is effective, it is costly, infrastructure-dependent, and vulnerable to disruption. Room-temperature dry storage, inspired by desiccation-tolerant organisms, provides an alternative by stabilizing biomolecules in vitrified ("glass-like") matrices that limit molecular motion which if not reduced can lead to breakdown and loss of integrity. Trehalose is widely used as a vitrifying agent, but its protective capacity depends on glassy properties shaped by drying methods, environmental conditions, storage duration, and the type of preserved molecule. Systematic studies linking these factors to short and long-term stability remain limited. Here, we examine how dryi

SUBMITTER: Kumara U 

PROVIDER: S-EPMC12871358 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature