Meiotic recombination and flanking marker exchange at the highly unstable human minisatellite CEB1 (D2S90).

ABSTRACT: Unequal crossover has long been suspected to play a role in the germline-specific instability of tandem-repeat DNA, but little information exists on the dynamics and processes of unequal exchange. We have therefore characterized new length alleles associated with flanking-marker exchange at the highly unstable human minisatellite CEB1, which mutates in the male germline by a complex process often resulting in the gene conversion-like transfer of repeats between alleles. DNA flanking CEB1 is rich in single-nucleotide polymorphisms (SNPs) and shows extensive haplotype diversity, consistent with elevated recombinational activity near the minisatellite. These SNPs were used to recover mutant CEB1 molecules associated with flanking-marker exchange, directly from sperm DNA. Mutants with both pro

SUBMITTER: Buard J 

PROVIDER: S-EPMC1287182 | biostudies-literature | 2000 Aug

REPOSITORIES: biostudies-literature