Combined transcriptomic and proteomic analysis reveals the response mechanisms of alfalfa to freezing stress.

ABSTRACT:

Introduction

Alfalfa (Medicago sativa L.) is the most important perennial forage crop cultivated globally. However, extreme environmental conditions, such as freezing stress, can significantly impact alfalfa's growth and development. The potential mechanisms through which alfalfa responds to freezing stress remain largely unexplored.

Methods

In this study, we analyzed the physiological indices, transcriptomes and proteomes of the cold-tolerant alfalfa cultivar "Dongnong NO.1" and the cold-sensitive cultivar "Bara 218TR" at -5°C.

Results

The results indicated that the levels of antioxidant enzyme and osmoregulatory substances in "Dongnong NO.1" were significantly higher than in "Bara 218TR". Additionally, the levels of malondialdehyde (MDA) and relative electrol

SUBMITTER: Ren N 

PROVIDER: S-EPMC12872475 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature