Heterologous expression of Thermomyces lanuginosus lipase in Aspergillus niger: stress resistance, optimization of fermentation conditions, and transcriptomic analysis.

ABSTRACT: Aspergillus niger serves as a cell factory for efficient enzyme production. The lipase derived from Thermomyces lanuginosus (TLL) is known for its remarkable thermal stability and is extensively utilized in various industrial fields. In this study, a heterologous expression strain, ΔAnTll-11, of TLL was successfully constructed in A. niger. Through fermentation optimization, the lipase activity was enhanced 8.7-fold to 4547.95 U/mL, compared with the initial value of 520 U/mL. Enzymatic characterization indicated that the recombinant lipase exhibited optimum activity at pH 9.5 and 45 °C, and its activity was positively influenced by Ca²⁺, Ag⁺, Mg²⁺, and Cu²⁺. The ΔAnTll-11 strain exhibited enhanced tolerance to high osmolarity, oxidative stress, and thermal

SUBMITTER: Shang X 

PROVIDER: S-EPMC12873016 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature