Prelamin A accumulation overlaps increased vascular calcification in peripheral artery disease and regulates vascular smooth muscle cells mineralization in diabetes mellitus.

ABSTRACT: This study investigated the mechanistic intersection between aging, diabetes mellitus (DM), and prelamin A accumulation in vascular calcification (VC) progression in patients with peripheral arterial disease (PAD) undergoing lower limb amputation. Arterial segments were collected from amputated PAD patients with (PAD + DM, n = 10) and without DM (PAD, n = 8), as well as from non-vascular amputated patients (CTRL, n = 3). Additional in vivo and in vitro experiments were conducted to investigate early-stages of VC and the impact of insulin resistance on prelamin A accumulation and ZMPSTE24 expression. PAD and PAD + DM patients exhibited greater VC than CTRL patients. Prelamin A accumulation was higher in PAD (6.3 ± 5.0 %) and PAD + DM (10.9 ± 6.4 %) than in CTRL (1.2 ± 1.3 %), while Z

SUBMITTER: Placencia EVD 

PROVIDER: S-EPMC12874100 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature