Three alternative promoters govern PGC-1α expression in chicken and are directly repressed by PPARγ.

ABSTRACT: Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a key transcriptional coactivator that orchestrates diverse physiological and pathological processes by interacting with factors such as PPARγ, PPARα, and NRFs. While extensively characterized in mammals, PGC-1α gene structure and regulation remain unexplored in chickens. Here, using 5' rapid amplification of cDNA ends (5' RACE), we identified three distinct first exons in chicken PGC-1α gene, indicative of three alternative promoters (P1, P2, and P3). Dual-luciferase reporter assays confirmed that all three alternative promoter regions displayed robust activity, exhibiting approximately 27.8-, 3.9-, and 6.0-fold higher reporter activity compared to the promoterless pGL3-Basic control vector, respectively. Bioinformati

SUBMITTER: Huang J 

PROVIDER: S-EPMC12874118 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature