Laser Desorption-Rapid Evaporative Ionization Mass Spectrometry (LD-REIMS): A New Tool for the High-Throughput Metabolomic and Lipidomic Profiling of Live Cells.

ABSTRACT: Understanding the dynamic cellular metabolism is essential for gaining deeper insights into inter- and intracellular functions. In recent years, mass spectrometry (MS) has become the technology of choice for the biochemical characterization and profiling of cell lines, particularly when coupled with separation techniques such as liquid chromatography (LC-MS). However, these methods typically involve extensive sample preparation with potent organic solvents, which is labor-intensive, time-consuming, and incompatible with direct analysis of intact, live cells. Here, we propose the use of the ambient ionization technique Laser Desorption-Rapid Evaporative Ionization Mass Spectrometry (LD-REIMS) incorporated in an automated platform, for the high-throughput profiling of live or frozen cell mon

SUBMITTER: Maneta-Stavrakaki S 

PROVIDER: S-EPMC12874226 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature