The Liquid State of RIM1α and RBP Condensates is Maintained by Lipids.

ABSTRACT: At the presynapse, RIM1α and RIM-binding protein (RBP) play a crucial role in regulating vesicle docking and priming. Emerging evidence suggests that these proteins are involved in the organization of the active zone where they cluster with membrane proteins and lipids, forming protein condensates driven by liquid-liquid phase separation (LLPS). While protein phase separation has been associated with cellular function, it has also been linked to various disorders, as liquid condensates can promote protein aggregation, leading to dysfunction. In this work, we investigated the phase behavior of RIM1α and RBP. We find that under physiological conditions and in the absence of crowding agents, RIM1α and RBP have the ability to spontaneously form biomolecular condensates. Moreover, these liquid

SUBMITTER: Fischer CM 

PROVIDER: S-EPMC12874637 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature