Identifying metabolic bottlenecks for micafungin precursor production via untargeted regulatory perturbation.

ABSTRACT:

Background

Micafungin, a clinically important echinocandin antifungal agent, is derived from the nonribosomal cyclic hexapeptide FR901379 produced by the filamentous fungus Coleophoma empetri. However, low fermentation efficiency remains a major constraint in its industrial production.

Results

In this study, we implemented an untargeted regulatory perturbation strategy to systematically identify metabolic bottlenecks affecting FR901379 biosynthesis. A mutant library was constructed by rationally engineering the key untargeted regulatory genes involved in histone modification and global regulation. The untargeted perturbation led to diverse phenotypes in both growth and secondary metabolism, ranging from enhancement (by up to 170%) to complete abolition of FR901379 production

SUBMITTER: Men P 

PROVIDER: S-EPMC12874660 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature