Discovery and design of novel glucoamylases suitable for raw starch hydrolysis at moderate temperatures via an integrated bioinformatics-assisted strategy.

ABSTRACT: Glucoamylases capable of hydrolyzing raw starch at moderate temperatures exhibit significant potential for the direct and efficient hydrolysis of raw starch. Using bioinformatics-assisted mining strategies, four candidate glucoamylases were initially identified from the GenBank database. Among these candidates, SeGA demonstrated high specific activities of 412.94 U/mg toward soluble starch and 143.51 U/mg toward raw corn starch. The half-life of SeGA was 192 h at 40 °C. Using SeGA as the initial enzyme, FuncLib design was performed to enhance its activity. The mutant SeGA-21 exhibited higher specific activity of 466.22 U/mg toward soluble starch and 173.98 U/mg toward raw corn starch, representing increases of 1.13-fold and 1.21-fold compared to SeGA, respectively. The half-life of SeGA-21

SUBMITTER: Ge S 

PROVIDER: S-EPMC12874731 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature