Autotrophic methylotrophy with no methanol dehydrogenase (MDH) in a strain of fluorescent <i>Pseudomonas</i>.

ABSTRACT:

Background

Very few true Pseudomonas methylotrophic strains have been described, and in none of them have the pathways for one-carbon (C₁) substrate metabolism been elucidated.

Methods

The genomes of three Pseudomonas strains able to grow on methanol as the sole source of carbon (C) and energy (E) were sequenced and analyzed, and one of the strains was further characterized at the proteomic and physiological level.

Results

None of the three strains possesses a classic methanol dehydrogenase enzyme, and they apparently employ generalist type-I alcohol dehydrogenases (ADHs) to catabolize methanol to formaldehyde. In two of the strains' genomes, the only complete route encoded for incorporating methylotrophic carbon is the Calvin-Benson-Bassham (

SUBMITTER: De Marco P 

PROVIDER: S-EPMC12875217 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature