A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.

ABSTRACT: In vitro spermatogenesis (IVS) remains a major challenge due to its complexity and extended duration. Although organ culture of neonatal mouse testes can support complete spermatogenesis, its three-dimensional structure limits precise observation and control of culture conditions, resulting in heterogeneous oxygen and nutrient gradients. Two-dimensional (2-D) systems provide greater accessibility but often induce accelerated or abnormal meiosis. In this study, we present a simplified, serum free 2-D culture system that enables meiotic progression of mouse germ cells to the mid pachytene stage. Testicular cells from Acrosin (Acr)-GFP transgenic mice (2.5 to 10.5 days postpartum: dpp) were enzymatically dissociated and cultured in APEL medium, a chemically defined and serum free basal formul

SUBMITTER: Yokoi Y 

PROVIDER: S-EPMC12875477 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature